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A Cellular FRET-Based Sensor for beta-O-GlcNAc, A Dynamic Carbohydrate Modification Involved in Signaling.

Carrillo LD, Krishnamoorthy L, Mahal LK

Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300 Austin, Texas 78712-0265.

&bgr;-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.

Published 15 November 2006 in J Am Chem Soc, 128(46): 14768-14769.
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