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Antifungal activity of chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9.

Nguyen NV, Kim YJ, Oh KT, Jung WJ, Park RD

Glucosamine Saccharide Materials, National Research Laboratory, Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, South Korea.

Two chitinolytic fungal strains, Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9, were isolated from soil samples of Korea and Vietnam, respectively. DY-59 and VS-9 crude chitinases secreted by these fungi in the 0.5% swollen chitin culture medium had an optimal pH of 4 and the optimal temperatures of 40 degrees C and 60 degrees C, respectively. Enzymatic hydrolysis products from crab swollen chitin were N-acetyl-beta-D-glucosamine (GlcNAc) by DY-59 chitinase, and GlcNAc and N, N'-diacetylchitobiose (GlcNAc)2 by VS-9 chitinases. The chitinases degraded the cell wall of Fusarium solani hyphae to produce oligosaccharides, among which GlcNAc, (GlcNAc)2, and pentamer (GlcNAc)5 were identified by high-pressure liquid chromatography. DY-59 and VS-9 chitinases inhibited F. solani microconidial germination by more than 70% and 60% at final protein concentrations of 5 and 27 microg mL(-1), respectively, at 30 degrees C for 20 h treatment.

Published 21 December 2007 in Curr Microbiol, 56(1): 28-32.
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