Glucosamine Research Today is a free monthly online journal that collates and summarizes the latest research about Glucosamine, including details on chondroitin sulfate, uses, effects, benefits, arthritis.

Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays.

Karamanska R, Clarke J, Blixt O, Macrae JI, Zhang JQ, Crocker PR, Laurent N, Wright A, Flitsch SL, Russell DA, Field RA

School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK.

Plant lectin recognition of glycans was evaluated by SPR imaging using a model array of N-biotinylated aminoethyl glycosides of beta-D-: glucose (negative control), alpha-D: -mannose (conA-responsive), beta-D-: galactose (RCA(120)-responsive) and N-acetyl-beta-D-: glucosamine (WGA-responsive) printed onto neutravidin-coated gold chips. Selective recognition of the cognate ligand was observed when RCA(120) was passed over the array surface. Limited or no binding was observed for the non-cognate ligands. SPR imaging of an array of 40 sialylated and unsialylated glycans established the binding preference of hSiglec7 for alpha2-8-linked disialic acid structures over alpha2-6-sialyl-LacNAcs, which in turn were recognized and bound with greater affinity than alpha2-3-sialyl-LacNAcs. Affinity binding data could be obtained with as little as 10-20 mug of lectin per experiment. The SPR imaging technique was also able to establish selective binding to the preferred glycan ligand when analyzing crude culture supernatant containing 10-20 mug of recombinant hSiglec7-Fc. Our results show that SPR imaging provides results that are in agreement with those obtained from fluorescence based carbohydrate arrays but with the added advantage of label-free analysis.

Published 8 February 2008 in Glycoconj J, 25(1): 69-74.
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